
The human body is a highly resilient system, able to heal itself in ways that can often seem like magic. But sometimes, the body needs help in certain places where healing may take longer, or places where the body does not see the need for healing at all. Examples of these circumstances can be a knee injury in which the area does not receive much blood flow, or loose skin on the face which is starting to wrinkle and sag. It has been made possible to heal orthopedic ailments as well as rejuvenate skin tissues with the use of Platelet Rich Plasma (PRP), a procedure that uses your own blood to repair you. Since blood has amazing healing properties for the body, this treatment has revolutionized the recovery time of joint and tendon injuries as well as the reversal of skin aging.
One of the crucial components of blood are platelets. In addition to carrying oxygen and nutrition, creating antibodies to ward against infections, and promoting clotting to stop blood loss, they support the body’s organs and tissues along with the red and white blood cells.
Concentrated platelets, which contain a high concentration of bioactive proteins and other growth factors that promote wound healing and tissue repair, are utilized in PRP therapy. The initial step involves extracting peripheral blood from the patient and preserving it with an anticoagulant, such as sodium citrate, to obtain the necessary sample for PRP injection.
The blood sample will subsequently undergo centrifugation. The platelets will be isolated from other blood cell and stem cell components using PRP centrifugation. This process should divide the blood into three layers:
Red blood cells are found in the bottom layer because of their higher density.
A thin, visible layer with a color range of yellow to white is produced by the middle layer. This layer contains the largest concentration of platelets and contains some leukocyte cells suspended among the platelets.
The platelet poor plasma, which has a low platelet concentration and is not advised for use in PRP treatment, is found in the uppermost layer.
Depending on the kit that your practice will use, there are three different methods for separating the blood into layers: buffy coat, gel separator, and buffy coat with double spin. The additional centrifugation in the buffy coat with double spin method aids in achieving complete isolation of autologous platelet-rich plasma, which can be easily extracted from the red blood cells.